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Effect of PRAK gene knockout on the proliferation of mouse embryonic fibroblasts
Xiaowei GONG MD, PhD, Xiaoyan MING MD, Xu WANG MM, Daan WANG MD, Peng DENG MM, Yong JIANG MD, PhD, Aihua LIU MD, PhD,
Frontiers of Medicine 2009, Volume 3, Issue 4, Pages 379-383 doi: 10.1007/s11684-009-0073-y
Keywords: p38 regulated/activated protein kinase gene knockout cell cycle cell proliferation
One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system
Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN
Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1, Pages 2-5 doi: 10.15302/J-FASE-2014007
Metabonomic study of the biochemical profiles of heterozygous myostatin knockout swine
Jianxiang XU,Dengke PAN,Jie ZHAO,Jianwu WANG,Xiaohong HE,Yuehui MA,Ning LI
Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 1, Pages 90-99 doi: 10.15302/J-FASE-2015045
Keywords: myostatin transforming growth factor-β family skeletal muscle metabolic disorders chromatography mass spectrometry metabonomics
Xuli Wang, Xin Xie, Jin Liu, Guo-Liang Wang, Dewen Qiu
Engineering 2020, Volume 6, Issue 5, Pages 546-552 doi: 10.1016/j.eng.2019.07.025
Reliable knowledge on pathogenic agents contributes to effective plant protection. For most plant pathogens, maintaining protein homeostasis (proteostasis) is essential for unfolding the cellular functions to survive and thrive. However, the fungal proteins involved in proteostasis remain poorly characterized in the process of pathogenesis. In this study, we characterized the function of the nascent polypeptide-associated complex (NAC) in Fusarium graminearum (F. graminearum, FgNAC), one of the top 10 fungal pathogens with predominant scientific/economic importance. We found that FgNACα, a subunit of FgNAC, manifests high structural and functional similarity to its homologous counterparts in yeast and other species. The mutants of F. graminearum lacking NACα are viable but suffer significant defects in vegetative growth, conidial production, and pathogenesis. In addition, we show here that FgNACα can interact with another subunit of NAC (FgNACβ) in a yeast-two-hybrid assay. The subcellular localization results show that FgNACα and FgNACβ are predominantly localized in the cytoplasm. Future studies should focus on deciphering the mechanism by which NAC orchestrates protein biogenesis and consequentially modulates development and pathogenesis.
Keywords: Fusarium head blight Nascent polypeptide-associated complex Gene knockout Pathogenicity Subcellular
Xia Jiahui
Strategic Study of CAE 2000, Volume 2, Issue 11, Pages 1-11
In this paper, the study on family collection, gene mapping, gene identification and gene functiont (1; 3) (q44; p11) associated with nasopharyngeal cancer was found in 1975 at first, and human TDF geneIn 1998, GJB3, a human genetic neurological deafness gene, was identified using a novel strategy of “Gene Family-Candidate Disease Gene Cloning”,and the paper was published in Nature Geneticswas also cloned from gene function study.
Keywords: genetic disease gene mapping and cloning gene familiy-candidate disease gene cloning genome wide scan gene function study
Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing
Yu Cao, Xiaoxuan Liu, Ling Peng
Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4, Pages 663-675 doi: 10.1007/s11705-017-1623-5
Keywords: gene therapy RNAi therapeutics dendrimer nanovectors gene silencing
The antibiotic resistome: gene flow in environments, animals and human beings
Yongfei Hu, George F. Gao, Baoli Zhu
Frontiers of Medicine 2017, Volume 11, Issue 2, Pages 161-168 doi: 10.1007/s11684-017-0531-x
Keywords: antibiotic resistance resistome microbiome gene flow
Reflections on the system of evaluation of gene-edited livestock
Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI
Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2, Pages 211-217 doi: 10.15302/J-FASE-2019303
Keywords: evaluation gene editing livestock performance safety
Frontiers of Medicine 2022, Volume 16, Issue 4, Pages 627-636 doi: 10.1007/s11684-020-0815-4
Keywords: RUNX1 gene mutation acute myeloid leukemia transcriptional repression DNA methylation
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
Frontiers of Medicine 2009, Volume 3, Issue 2, Pages 141-147 doi: 10.1007/s11684-009-0041-6
Keywords: Rab9 lentivirus gene therapy gene transfer
Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng
Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4, Pages 521-528 doi: 10.1007/s11705-017-1612-8
Keywords: α-helical polypeptide plant gene delivery protoplast intact leaves transfection
Profiling influences of gene overexpression on heterologous resveratrol production in
Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan
Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1, Pages 117-125 doi: 10.1007/s11705-016-1601-3
Keywords: resveratrol aromatic amino acid DNA assembly metabolic engineering gene overexpression
Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity
Chuanfeng Wu, Cynthia E. Dunbar
Frontiers of Medicine 2011, Volume 5, Issue 4, Pages 356-371 doi: 10.1007/s11684-011-0159-1
Keywords: gene therapy hematopoietic stem cells insertional mutagenesis genotoxicity induced pluripotent stem cell
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen
Frontiers of Medicine 2015, Volume 9, Issue 1, Pages 90-99 doi: 10.1007/s11684-015-0390-2
Gene therapy provides a potential cure for hemophilia B, and significant progress has been achievedin liver-directed gene transfer mediated by adeno-associated viral vectors.vectors and hFIX expression cassette may positively contribute to the ultimate success of hemophilia B geneIn addition, the regulatory elements that control the FIX gene expression in these cassettesreconstructs of hFIX with AAV vector may potentially contribute to the creation of an efficacious gene
Keywords: factor IX hemophilia B liver-specific regulatory elements hydrodynamic gene transfer
Lu Wang
Frontiers of Medicine 2013, Volume 7, Issue 3, Pages 280-289 doi: 10.1007/s11684-013-0265-3
Many gene fusions have been recognized as important diagnostic and/or prognostic markers in humanIn recent years, novel gene fusions have been identified in cases without prior knowledge of the geneticThis review focused on advanced genome-wide screening approaches in fusion gene identification, suchas microarray-based approaches, next-generation sequencing, and NanoString nCounter gene expression systemThe fundamental rationale and strategy for fusion gene identification using each biotech platform are
Keywords: gene fusion cancer microarray next-generation sequencing NanoString nCounter system
Title Author Date Type Operation
Effect of PRAK gene knockout on the proliferation of mouse embryonic fibroblasts
Xiaowei GONG MD, PhD, Xiaoyan MING MD, Xu WANG MM, Daan WANG MD, Peng DENG MM, Yong JIANG MD, PhD, Aihua LIU MD, PhD,
Journal Article
One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system
Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN
Journal Article
Metabonomic study of the biochemical profiles of heterozygous myostatin knockout swine
Jianxiang XU,Dengke PAN,Jie ZHAO,Jianwu WANG,Xiaohong HE,Yuehui MA,Ning LI
Journal Article
Nascent Polypeptide-Associated Complex Involved in the Development and Pathogenesis of Fusarium graminearum on Wheat
Xuli Wang, Xin Xie, Jin Liu, Guo-Liang Wang, Dewen Qiu
Journal Article
Study on Family Collection, Gene Mapping, Gene Identification and Gene Function of Human Genetic Diseases
Xia Jiahui
Journal Article
Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing
Yu Cao, Xiaoxuan Liu, Ling Peng
Journal Article
The antibiotic resistome: gene flow in environments, animals and human beings
Yongfei Hu, George F. Gao, Baoli Zhu
Journal Article
Reflections on the system of evaluation of gene-edited livestock
Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI
Journal Article
Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation
Journal Article
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
Journal Article
Gene delivery into isolated
Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng
Journal Article
Profiling influences of gene overexpression on heterologous resveratrol production in
Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan
Journal Article
Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity
Chuanfeng Wu, Cynthia E. Dunbar
Journal Article
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen
Journal Article